Two Proton Pump Interactors Identified from a Direct Phosphorylation Screening of a Rice cDNA Library by Using a Recombinant BRI1 Receptor Kinase
スポンサーリンク
概要
- 論文の詳細を見る
A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.
- 日本植物細胞分子生物学会の論文
- 2004-03-01
著者
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ABE Hiroshi
Faculty of Engineering Sciences, Kyushu University
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Kasahara Masahiro
Gene Research Center Tokyo University Of Agriculture And Technology
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Nyunoya Hiroshi
Gene Res. Center Tokyo Univ. Of Agr. & Tech.
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Matsushita Yasuhiko
Gene Res. Center Tokyo Univ. Of Agr. & Tech.
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Matsushita Yasuhiko
Gene Research Center Tokyo University Of Agriculture And Technology
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Sato Michio
Gene Research Center Tokyo University Of Agriculture And Technology
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HIRABAYASHI Shuichi
Gene Research Center, Tokyo University of Agriculture and Technology
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OH-I Rie
Gene Research Center, Tokyo University of Agriculture and Technology
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Abe H
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Oh-i Rie
Gene Research Center Tokyo University Of Agriculture And Technology
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Hirabayashi Shuichi
Gene Research Center Tokyo University Of Agriculture And Technology
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Abe Hiroshi
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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