Molecular approaches for producing low-phytic-acid grains in rice

概要

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Phytic acid (myo-inositol hexakisphosphate) is the storage form of phosphorus in plant seeds. Reduction of phytic acid levels in seeds leads to both an increase in the availability of phosphorus and a reduction in the environmental load. Myo-inositol-1-phosphate (Ins(3)P1) synthase (EC 5.5.1.4) catalyzes the first step in phytic acid biosynthesis. We attempted to reduce the phytic acid content of seeds through manipulation of the expression of the Ins(3)P1 synthase gene. A rice Ins(3)P1 synthase gene, RINO1, was transformed into rice plants in the antisense or sense orientation under the control of the CaMV 35S promoter or the RINO1 promoter. Some T1 seeds that transformed both with RINO1 promoter::antisense RINO1 gene and with 35S promoter::sense RINO1 gene increased the amount of inorganic phosphates, as compared to those of non-transgenic plants. This result suggested a molar-equivalent decrease in phytic acid. We also examined RINO1 promoter activity using a β-glucuronidase (GUS) reporter gene. Finally, we discuss the strategy of molecular breeding to reduce phytic acid levels in seeds.
日本植物細胞分子生物学会の論文
2004-09-01