Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit

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概要

• 論文の詳細を見る

• Somatic hybrids were produced between embryogenic callus protoplasts of ‘Shogun’ mandarin (Citrus reticulata Blanco, 2n = 2x = 18) and leaf protoplasts of grapefruit (C. paradisi Macf., 2n = 2x =18) by electrofusion. Hybridity of the two regenerated plants was confirmed by leaf characteristics and nuclear genome analysis using RAPD and PCR-RFLP. Flow cytometry and root-tip cell count of the two hybrids revealed a chromosome number of 27. These triploid somatic hybrids on transfer to field showed normal morphology and vigorous growth. Field performances as well as disease resistance are under investigation.

• 日本植物細胞分子生物学会の論文
• 2002-12-03

著者

• Komatsu Haruki

  Laboratory Of Pomology School Of Agriculture Kyushu Tokai University

• KUNITAKE Hisato

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• NAGASAWA Kohji

  Laboratory of Pomology, School of Agriculture, Kyushu Tokai University

• TAKAMI Kayo

  Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University

• Takami Kayo

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

• Nagasawa Kohji

  Laboratory Of Pomology School Of Agriculture Kyushu Tokai University

• Kunitake Hisato

  Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University

関連論文

• Molecular and Cytogenetic Characterization of Triploid Somatic Hybrids between 'Shogun' Mandarin and Grapefruit

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