Characterization of DNA methyltransferase genes in Brassica rapa

スポンサーリンク

概要

• 論文の詳細を見る

• DNA methylation is essential for normal development and plays important roles in regulating gene expression in plants. Analysis of the key enzymes catalyzing DNA methylation is important to understand epigenetic phenomena. In this study, three putative methyltransferase genes, BrMET1a, BrMET1b, and BrCMT, were isolated from a genome library of Brassica rapa. Structural conservation of the amino acid sequence between BrMET1a/BrMET1b and AtMET1 and that between BrCMT and AtCMT3 suggests that they may function as DNA methyltransferase. BrMET1a was expressed in vegetative and reproductive organs, while BrMET1b was expressed only in pistils, indicating that these two genes have different functions. BrCMT was expressed especially in stamens at the stage of 2–4 days before anthesis. We isolated three DNA methyltransferase genes in Brassica rapa and indicated differences of expression patterns of these DNA methyltransferase genes and expression levels in different tissues and developmental stages, suggesting that these genes might play important roles in epigenetic gene regulation in B. rapa.

• 日本遺伝学会の論文
• 2006-08-25

著者

• Sasaki Taku

  Graduate School Of Agricultural Science Tohoku University

• Nishio Takeshi

  Graduate School Of Agric. Sci. Tohoku Univ.

• FUJIMOTO Ryo

  Graduate School of Agricultural Science, Tohoku University

• Fujimoto Ryo

  Graduate School Of Agricultural Science Tohoku University

関連論文

• Development of a Novel Breeding Method Using SNP-based Selection of Rice Genotypes(Frontiers in Rice Science -from Gene to Field-,The 100^ Anniversary of Tohoku University, International Symposium)
• Dot-blot Analysis for Identification of japonica Rice Cultivars and Genotyping of Recombinant Inbred Lines
• Identification of 205 current rice cultivars in Japan by dot-blot-SNP analysis
• Mutant Selection from Progeny of Gamma-ray-irradiated Rice by DNA Heteroduplex Cleavage using Brassica Petiole Extract
• Molecular characterization of mutations induced by gamma irradiation in rice
• Fluorogenic Ribonuclease Protection (FRIP) Analysis of Single Nucleotide Polymorphisms (SNPs) in Japanese Rice (Oryza sativa L.) DNA for Cultivar Discrimination
• Knockout of glutelin genes which form a tandem array with a high level of homology in rice by gamma irradiation
• New Rice Varieties with Low Levels of Easy-to-digest Protein, 'LGC-Katsu' and 'LGC-Jun'
• The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species
• Characterization of DNA methyltransferase genes in Brassica rapa
• The Molecular Mechanism of Self-Recognition in Brassica Self-Incompatibility
• Analysis of QTL for lowering cadmium concentration in rice grains from 'LAC23'
• Interspecific Pairs of Class II S Haplotypes Having Different Recognition Specificities between Brassica oleracea and Brassica rapa
• Small variation of glucosinolate composition in Japanese cultivars of radish (Raphanus sativus L.) requires simple quantitative analysis for breeding of glucosinolate component
• Close linkage of a blast resistance gene, Pias(t), with a bacterial leaf blight resistance gene, Xal-as(t), in a rice cultivar 'Asominori'

もっと見る 閉じる

スポンサーリンク