Rapid construction of Drosophila RNAi transgenes using pRISE, a P-element-mediated transformation vector exploiting an in vitro recombination system
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概要
- 論文の詳細を見る
RNAi is a gene-silencing phenomenon mediated by double-stranded RNA (dsRNA) and has become a powerful tool to elucidate gene function. To accomplish rapid construction of transgenes expressing dsRNA in Drosophila, we developed a novel transformation vector, pRISE, which contains an inverted repeat of the attR1-ccdB-attR2 cassette for in vitro recombination and a pentameric GAL4 binding site for conditional expression. These features enabled us to construct RNAi transgenes without a complicated cloning scheme. In cultured cells and transgenic flies, pRISE constructs carrying dsRNA transgenes induced effective RNAi against an EGFP transgene and the endogenous white gene, respectively. These results indicate that pRISE is a convenient transformation vector for studies of multiple Drosophila genes for which functional information is lacking.
- 日本遺伝学会の論文
- 2006-04-25
著者
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Kondo Takefumi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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INAGAKI Sachi
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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YASUDA Kunio
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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KAGEYAMA Yuji
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Yasuda Kunio
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Inagaki Sachi
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kageyama Yuji
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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