ヒトのアルコ-ル脱水素酵素β1をコ-ドするcDNAクロ-ンの構造解析と他のクラス1サブユニットαやγとの進化的関係〔英文〕

概要

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Three different sizes of cDNA clones coding for the β1 subunit of human alcohol dehydrogenase (ADH) were isolated from a human cDNA library constructed in bacteriophage λgtll and completely sequenced. Comparisons of these three with two other published ADH β1 cDNA sequences show one silent nucleotide substitution at the third position of colon 220 and two nucleotide differences within the 1337 nucleotides of the 3′ untranslated region. Hybridization of a 3′ untranslated region of one of the β1 cDNA clones to DNAs from various individuals has shown a Rsa I restriction fragment length polymorphism. A phylogenetic tree for class I human ADHs α, β, and γ shows that the subunits α and diverged most recently and that their common ancestor diverged from the ancestor of the subunit γ earlier. This tree topology differs from that based on equal rates of nucleotide (or amino acid) substitution of the three ADH subunits, in which the subunits β and γ are considered to be most recently diverged. The evolutionary rates of nucleotide substitution for the three subunits reveal that the subunit γ is evolving with the slowest rate, followed by β and γ, in that order, implying that the subunit γ may be providing the original function of ethanol metabolism.
日本遺伝学会の論文