大腸菌recA株の作成法〔英文〕

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概要

• 論文の詳細を見る

• We isolated mutations in cys genes located in close vicinity of recA gene in Escherichia coli, by insertion of Tn1, Tn5 or Tn1O. We deviced a simple method constructing recA strains using these mutations. The cys mutations were transduced by P1kc into an appropriate recipient selecting for drug resistance of the mutations. The resultant Cys- transductants were crossed with an Hfr RecA- strain, KL16-99, and Cys+ recombinants were picked up. Among them, small colony formers were Rec- strains.

• 日本遺伝学会の論文

著者

• HARAYAMA Shigeaki

  Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo

• IINO Tetsuo

  Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo

関連論文

• 大腸菌recA株の作成法〔英文〕

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