ソバカルスの継代培養からえられた復元植物〔英文〕

概要

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For callus induction and restoration of plants from callus tissue of buckwheat, kinetin showed no effect, but supplements of 2, 4-D (10mg/l) to White's basal medium induced calluses from germinating seeds. Callus tissues subcultured for 48 months grew vigorously on the modified White's basal medium.Nearly 40.5% of cells of callus tissues which were subcultured 6-36 months had the diploid chromosome number 16. The cell population became much more uniform in chromosome number after a longer period of subculture.Shoot and root formation were not found on the proliferating calluses in White's basal medium and modified white's basal medium. However, transfer to modified RM-1964 medium after one or two months resulted in a tendency towards organ-forming capacity.The five restored plants obtained showed normal chromosome constitution.
Genetics Society of Japanの論文