型質転換による枯草菌のアミラーゼ遺伝子の微細構造の研究〔英文〕

概要

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To make genetic analysis of the amylase gene (amy) of Bacillus subtilis, selective markers linked to amy were looked for. As the result, phe96 (phenylalanine) and aro116 (aromatic amino acids) were found to be linked to amy. The two mutations were closely linked each other.By use of the linked markers, fine mapping of amylase negative mutations was carried out by transformation. Sixteen amylase negative mutations so far isolated were mapped in a single locus.
日本遺伝学会の論文