キイロショウジョウバエにおける異種のメラニン腫瘍についての生化学的研究〔英文〕
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概要
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Protein fractions of the Drosophila larva in tu and Oregon R strains by means of an ammonium sulfate were studied by Tiselius-electrophoresis and by ultraviolet analysis, and it was found that a qualitative difference between tu and the control was electro-phoretically observed in a fraction (F-3) of five fractions obtained from the 12000g.- supernatant of larva-homogenates treated with an ammonium sulfate (2.8M in final concentration); namely, the tu material has only one peak in a direction “up” although the control shows two little peaks. A quantitative difference of ultraviolet-absorption at around 285mμ was also observed between tu and the control in a fraction (F-2).On the other hand, tumor-inducibility of “Freckled-type” melanotic tumor, F-type, was detected by injection of three fractions (F-1, F-2, and F-3) separated from these larva-homogenates by DEAE-cellulose column-chromatography (NaCl-linear concentration gradient-elution), into both pre-third instar larva of Varese (non-tumor strain) and Chieti-v (a strain with low tumor incidence of ordinal melanotic tumor, M-type). The results showed that the inducibility of F-type tumor in Varese and the relatively higher inducibility of M-type tumor might be in the F-2. Further, the F-2 was checked for the existence of nucleic acids by ultraviolet analysis, orcinol and diphenylamine reactions, and 32Pi-incorporation experiment. However, negative results were obtained. From these experimental results and the previous reports (Kanehisa 1960b), it is suggested that the F-type tumor-inducible factor (or factors) may not be a substance with nucleic acids but a kind of biochemically active protein which contains tryptophan or its derivative, and that such a specific factor may not specifically be different from a factor for M-type tumor and its specificity for F-type tumor may depend on hereditary nature of the host-itself responsible for the factor.
- 日本遺伝学会の論文
著者
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KANEHISA T.
Biological Laboratory, Faculty of Science, Kobe University
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FUJII S.
Biological Laboratory, Faculty of Science, Kobe University