イエバエにおけるエステラーゼの遺伝学的研究〔英文〕

概要

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Soluble esterases which hydrolyse β-naphthylacetate have been prepared from the 10 laboratory strains of the house fly and separated into 24 electrophoretic bands by Ogita's thin layer electrophoretic technique. First, these esterase bands were distinguished by electrophoretic mobilities. They were classified into enzymatic families on their behavior differences. The differences included changes in the pattern of esterases during ontogeny, and the heterogeneity with regard to substrate specificity and inhibitor susceptibility as well as the localization of esterases in various tissues. Linkage tests have shown that one of the bands might be controlled by a genetic factor on the 4th chromosome, and the others by genetic factors on the 5th chromosome. Genetic analysis of these esterase bands indicates that the loci responsible for the two esterases are located on the 5th chromosome. They were named as Est-A and Est-B loci. A recombination value between the cm and Est-A loci is approximately 20.7±and that between the cm and Est-B loci is 50.6±mapunits.The esterase bands which are active upon naphthylacetate esters are not correlated with the diazinon-resistance gene.
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