ソラルソンのHeLaおよびHL-C細胞におよぼす影響(腫瘍におよぼす薬物の細胞学的研究〔英文〕-24-)
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概要
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To detect at least one desirable mutant with a minimum total number of X2-plants or of combined X1- and X2-plants, two methods were established as suitable in relation to tillers of X1-plants.They are: a type of the "one-plant-two-grain method" (method D2) and of the "one-plant-three-grain method" (method E3) in which one grain is taken from each of two or three heads of an X1-plant respectively.These methods have been evaluated by considering the probability that one or more plants with the mutant allele in homozygous recessive form will appear in the total X2-plants in relation to the number of heads per X1-plant and to the independent degrees of mutation among the heads of the X1-plant.The "one-plant-one-grain method" (method C) proposed by Yoshida (1962) is the best when the number of heads per X1-plant (t) is equal to or smaller than three and method C or D2 is the best when t_??_4.No single method is known to cover all of the advantages. However, methods D2 and E3 will be better than the "one-plant-two-grain method" and the "one-plant-three-grain method" proposed in the first report of this series when t_??_2 and t_??_3 respectively. Generally speaking, it does not seem advantageous to use the "one-ear-one-grain method". especially the "one-ear-two-grain method" and the "one-ear-three-grain method".
- 日本遺伝学会の論文