Characterization of telomeres in Hordeum vulgare chromosomes by in situ hybridization-1-Normal diploid barley〔英文〕

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概要

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• Telomeres are the physical ends of eukaryotic chromosomes. In order to characterize the telomeres of barley (Hordeum vulgare L.), in situ hybridization to somatic metaphase chromosomes was conducted, using the cloned telomere sequence from Arabidopsis thaliana. The results showed hybridization signals at both ends of all 14 chromosomes. No hybridization signals at interstitial sites were observed. These results indicate that the telomere sequences of Arabidopsis are also found on the ends of barley chromosomes. The hybridization signals were observed to vary in size between arms of the same chromosomes, indicating differences in copy numbers of the telomeric repeat. With in situ hybridization, the barley telomeres became physically visible under the light microscope.

• 日本遺伝学会の論文

著者

• Wang Shaoke

  Department of Agronomy, Colorado State University Fort Collins

• Lapitan Nora

  Department of Agronomy, Colorado State University Fort Collins

• Tsuchiya Takumi

  Department of Agronomy, Colorado State University Fort Collins

• WANG Shaoke

  Department of Agronomy Colorado State University

関連論文

• Characterization of telomeres in Hordeum vulgare chromosomes by in situ hybridization-1-Normal diploid barley〔英文〕
• Cytogenetics of Ditelotetrasomic for Short Arm of Chromosome 3 of Barley (Hordeum vulgare L.)

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