黄花ルーピンにおけるアルカロイド反応の消長と変化〔英文〕
スポンサーリンク
概要
- 論文の詳細を見る
1. The present paper deals with the phenotypical and genotypic changes of alkaloid reaction in yellow lupine. Samples were got from the four types derived from the cross between common and sweet strains.2. A simple method for alkaloid testing was devised. That is, many leaves were maintained in a thermostat running at 60°C. Half-dried, but still green, leaves were then soaked in a solution of iodine potassium iodide. If the material turns brown in 0.5-1 hour, it proves positive in alkaloid reaction. Another device was a quantitative analysis by means of the dilution method, using an original extraction from fresh leaves with 5% HCl (Table 1, Fig. 1).3. Alkaloid reaction was ascertained to vary with leaf position, showing remarkably at upper young ones (Table 2). The reaction was found in almost equal grade up to the flowering stage from young one, but it appreciably dropped down at pod-formation stage (Table 3). Moreover, the degree of reaction varied also with kinds of organs, and the reaction was strong in seeds and anthers. Even in sweet strain, the reaction in seeds occurred considerably strong, and so in cotyledons and anthers (Table 4). Furthermore, by the injury given to the plant the reaction became strong and in sweet strain it occurred also (Table 5).4. Using both of simple and usual methods, many of leaves were tested and some variants found (Table 6). By the results of those investigations, most of the progeny of variants which changed to positive reaction (+) segregated roughtly into a ratio of 3:1, but few variants changed to negative reaction (-) bred true hereafter (Table 7).5. Another variant with yellow mosaic admidst the alkaloid-negative stock was found. This is characterized by having weakly positive leaves and mosaic yellow in parts of stem, leaf and pod (Fig. 2). The progeny segregated into green and yellow. Green one, which is alkaloid-negative, bred true. Yellow one kept on splitting into green and yellow (Table 8). From the consideration of this result and F1 features (Table 9) it may be assumed that the yellow character behaved as dominant to the green one and showed heterozyous condition. Moreover the degree of alkaloid reaction of fresh leaves in yellow variant was about one-sixth the amount in common strain and double of that of alkaloid-negative green (Table 10).
- 日本遺伝学会の論文
著者
-
NAGATOMO Takashi
Laboratory of Plant Breeding, Faculty of Agriculture, Miyazaki University
-
片山 義勇
Laboratory of Plant Breeding, Faculty of Agriculture, Miyazaki University