Solanum chacoense×S.phureja雑種集団におけるRandom amplified polymorphic DNAマ-カ-〔英文〕

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概要

• 論文の詳細を見る

• Reproducible random amplified polymorphic DNA (RAPD) patterns were obtained in two potato species (Solanum chacoense and S. phureja), one of their F1s, and its derived F2 population, by adjusting temperature profiles of the amplification process and template DNA concentrations (1 ng/μl reaction vol.). Although the number of amplified products and detectable differences between the two species increased with increasing GC content of the primer, 50% or 60% GC is recommended for maximizing scorable RAPDs in the F2 population. Using 82 primers, 589 RAPDs were detected between the parents, and 70% of them (409 RAPDs) in the F1 clone. The number of RAPDs reliably scored for their segregations in the F2 population was significantly lowered because of complicated RAPD patterns. Consequently, 22% of the RAPDs detected in the parents (129 RAPDs), an average of 1.57 RAPDs per primer, were obtained as genetic markers, which can be used for the construction of a genetic map for this particular population.

• 日本遺伝学会の論文
• 1994-02-25

著者

• Hosaka Kazuyoshi

  Experimental Farm Kobe University

• Hosaka K

  Experimental Farm Kobe University

• HANNEMAN Robert

  USDA, ARS, Department of Horticulture, University of Wisconsin

• Hanneman Robert

  Usda Ars Department Of Horticulture University Of Wisconsin

関連論文

• 日本産バレイショのRAPD法による品種識別〔英文〕
• Random amplified polymorphic DNAs(RAPDs)を利用したイネの品種識別〔英文〕
• 日本と欧州におけるバレイショ葉緑体DNA型の変遷〔英文〕
• Solanum chacoense×S.phureja雑種集団におけるRandom amplified polymorphic DNAマ-カ-〔英文〕

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