大腸菌rhlE遺伝子の構造解析〔英文〕

スポンサーリンク

概要

• 論文の詳細を見る

• The E. coli chromosome is known to carry at least five genes, each of which codes for a "D-E-A-D" box protein that is presumed to possess an ATP-dependent RNA helicase activity. Four of such genes (srmB, deaD, dbpA and rhlB) were already mapped on the E. coli chromosome and their DNA sequences determined. We here report the complete nucleotide sequence of the remaining rhlE gene located at about 17.8 min on the E. coli genetic map. RhlE protein possesses all of the motifs (I to VI) conserved among prokaryotic and eukaryotic "D-E-A-D" proteins and has an arginine-rich carboxyl-terminal region. A null mutant of the rhlE gene was constructed by a new method with a ColE1 plasmid mutant that replicates in RNase HI-deficient bacterial strains, but not in the wild-type strains. The ΔrhlE mutant can grow normally, implying that the rhlE gene product is nonessential for bacterial cell growth.

• 日本遺伝学会の論文
• 1994-02-25

著者

• Ohmori Haruo

  Institute For Virus Research Kyoto University

関連論文

• The Escherichia coli ldcC gene encodes another lysine decarboxylase, probably a constitutive enzyme
• Characterization of the Y family of DNA polymerase in Drosophila
• Isolation and Characterization of UmuC/DinB/Rev1/Rad30 Family in Drosophila
• 2P-006 ヒト由来REV7-REV3複合体のX線結晶構造解析(蛋白質-構造,第47回日本生物物理学会年会)
• 2P-017 ヒトPCNA G178S変異体の構造研究(蛋白質・構造(2),第46回日本生物物理学会年会)
• 1P023 Structural insight of interaction between proliferating cell nuclear antigen and human TLS polymerases(1. Protein structure and dynamics (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• 大腸菌rhlE遺伝子の構造解析〔英文〕

スポンサーリンク