キイロショウジョウバエにおけるα-アミラ-ゼ遺伝子発現に関与するシス調節因子の分子解析--アミラ-ゼproximal遺伝子発現におけるアミラ-ゼdistal遺伝子上流の短い領域の必要性〔英文〕
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概要
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The structural locus for a-amylase (AMY) in Drosophila melanogaster is duplicated and divergently transcribed. These two genes are designated as Amy-p and Amy-d, respectively. We searched for the cis-acting regulatory elements for full expression of the duplicated Amy-p and Amy-d loci, by injecting plasmid constructs containing sequences from the Amy locus into preblastoderm embryos of an AMY-null strain and measuring exogenous AMY activity produced in transformed host larvae (i.e., the transient expression assay). Relative activities of endogenous amylase isozymes, AMY-1 and AMY-3, in extracts of AMY1,3 larvae of a Canton-S are almost the same. However, three independently isolated Amy-p1 constructs with only the 5 upstream regions of Amy-p1 expressed a very low AMY-1 activity. Two other Amy-p1 constructs with the 5 upstream region of Amy-d3 in addition to that of Amy-p1 produced a high activity. Thus, the 5 upstream region of Amy-d3 is necessary for full expression of Amy-p1. In order to locate cis-regulatory elements within the 5 region of Amy-d3, a series of hybrid constructs including this region were tested to locate them. Our results clearly show that the cis-acting regulatory sequences required for full expression of Amy-p1 are located between the base pairs at -304 and -372 upstream of Amy-d3 gene. In other words, only a short region located upstream of Amy-d 3 was found to be necessary and sufficient for the full expression of Amy-p1 in addition to its promoter. This region seems also necessary for the expression of Amy-d 3.
- 日本遺伝学会の論文
- 1994-12-01
著者
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山崎 常行
Department Of Biology Faculty Of Science Kyushu University
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Yamazaki Tsuneyuki
Department Of Biology Faculty Of Science Kyushu University
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CHOI Jeohg
Department of Biology, Faculty of Science, Kyushu University
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Choi Jeohg
Department Of Biology Faculty Of Science Kyushu University
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崔 貞仁
Department of Biology, Faculty of Science, Kyushu University
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