Acceleration of Lipid Peroxidation in α-Tocopherol Transfer Protein-Knockout Mice Following the Consumption of Drinking Water Containing a Radical Initiator

スポンサーリンク

概要

• 論文の詳細を見る

• To assess the antioxidative role of vitamin E (VE) in a mouse model of severe VE deficiency by using biomarkers, α-tocopherol transfer protein (α-TTP-/-)-knockout mice were maintained on a VE-deficient diet for 28 weeks [KO group, n = 6]. Wild-type C57BL/6 mice were maintained on a diet containing 0.002% α-tocopherol [WT group, n = 6]. The animals were housed individually in a metabolic cage from the age of 9 weeks (Week 0) to 27 weeks. Urine was collected every week, and the levels of total hydroxyoctadecadienoic acid (tHODE), 7-hydroxycholesterol (t7-OHCh), and 8-iso-prostaglandin F2α(t8-isoPGF2α), which are biomarkers for lipid peroxidation, were measured by gas chromatography (GC)-mass spectrometry. From the age of 21 weeks (Week 12), three mice in each group were provided drinking water containing the water-soluble radical initiator 2,2′-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (AIPH) until the end of the study (Week 19). Blood and tissue samples were collected, and the levels of the abovementioned biomarkers therein were assessed. AIPH consumption clearly elevated the plasma and erythrocyte levels of tHODE and t8-isoPGF2α in both the WT and KO groups except for the erythrocyte level of tHODE in the WT group. Furthermore, this elevation was more prominent in the KO group than in the WT group. Interestingly, AIPH consumption reduced the stereoisomer ratio of HODE (ZE/EE), which is reflective of the efficacy of a compound as an antioxidant in vivo; this suggests that free radical-mediated oxidation reduces the antioxidant capacity in vivo. The urine levels of tHODE, t7-OHCh, and t8-isoPGF2α tended to increase with AIPH consumption, but these individual levels fluctuated. It was clearly demonstrated by the proposed biomarkers that maintaining α-TTP-/- mice on a VE-deficient diet results in a severe VE deficiency and promotes lipid peroxidation.

• 日本油化学会の論文

著者

• Hayakawa Mieko

  Health Technology Research Center, National Institute of Advanced Industrial Science and Technology

• Yoshida Yasukazu

  Health Technology Research Center National Institute Of Advanced Industrial Science And Technology (

• Yoshida Yasukazu

  徳島大学医学部

• Niki Etsuo

  Health Research Institute National Institute Of Advanced Industrial Science And Technology

• Cynshi Osamu

  Fujigotemba Research Laboratories, Chugai Pharmaceutical Co. Ltd.

• Jishage Kou-ichi

  Fujigotemba Research Laboratories, Chugai Pharmaceutical Co. Ltd.

• Yoshida Yasukazu

  Health Research Institute National Institute Of Advanced Industrial Science And Technology

• Hayakawa Mieko

  Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST)

関連論文

• DPE-030 Serum Concentrations of Linoleic Acids and Their Derivatives by Lipid Peroxidation are Increased in Patients with CD36 Deficiency(DPE05,Lipid Disorders (H),Digital Poster Session (English),The 73rd Annual Scientific Meeting of The Japanese Circula
• Evaluation of the Antioxidant Effects of Coffee and Its Components Using the Biomarkers Hydroxyoctadecadienoic Acid and Isoprostane
• 3TA3-06 酸化ストレスによる細胞サイズリポソームの動的構造変化(生体膜・人工膜-構造・物性,第47回日本生物物理学会年会)
• Assessment of the Antioxidant Capacity of Natural Fruit Extracts by Inhibition of Probe Decay and Plasma Lipid Peroxidation
• Evaluation of Acute Oxidative Stress Induced by NiO Nanoparticles In Vivo and In Vitro
• Acceleration of Lipid Peroxidation in α-Tocopherol Transfer Protein-Knockout Mice Following the Consumption of Drinking Water Containing a Radical Initiator
• In vitro evaluation of cellular responses induced by stable fullerene C_ medium dispersion
• Biomarkers for oxidative stress : measurement,validation,and application
• Cellular responses induced by cerium oxide nanoparticles : induction of intracellular calcium level and oxidative stress on culture cells

スポンサーリンク