下垂体糖タンパク質ホルモン共通のα鎖と、FSHおよびLHβ鎖の、エルクにおけるcDNAのクローニング

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概要

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• We report the nucleotide and deduced amino acid sequences of the pituitary glycoprotein hormone common α-subunit, and the FSH and LH β-subunits of the elk (Cervus elaphus). These sequences were obtained by RT-PCR of pituitary gland polyadenylated RNA. A partial genomic fragment of the elk follicle stimulating hormone β-subunit was also amplified from genomic DNA and sequenced. These sequences show high similarity to other related domestic species, including sika deer, ovine, bovine, porcine and equine. The presently reported cDNAs were used as probes for Northern analysis of pituitary gland and muscle samples from elk and sheep. Mature mRNA transcripts of approximately 700, 1700 and 550 bases were expressed in the pituitary gland but not in muscle tissue, for the glycoprotein hormone common α-subunit and FSH and LH β-subunits, respectively. These observations are consistent with reports for other related species. The genomic fragment of elk FSH β-subunit revealed an intronic microsatellite of 6 CT repeats, which differs from the 19 CT repeats documented for the sheep genomic sequence. We propose that differences in the CT repeats of the elk FSH β-subunit gene may be used as a marker for elk parentage testing. The elk DNA sequences presently reported represent a unique addition to the growing collection of the pituitary hormone genes for various vertebrates, which will facilitate evolutionary and phylogenetic studies of the gonadotropin genes. In addition, this information and the cloned cDNAs will be useful for studying seasonal expression of the elk pituitary glycoprotein genes.

• 日本繁殖生物学会の論文

著者

• Clark Rena

  Department Of Veterinary Biomedical Sciences University Of Saskatchewan

• Chedrese P.

  Department Of Obstetrics Gynecology And Reproductive Sciences Royal University Hospital

• Furlan Michael

  Department Of Obstetrics Gynecology And Reproductive Sciences University Of Saskatchewan

• FURLAN Micheal

  Department of Obstetrics, Gynecology and Reproductive Sciences, University of Saskatchewan, Royal Un

• CLARK Rena

  Department of Veterinary Biomedical Sciences, Gynecology and Reproductive Sciences, University of Saskatchewan, Royal University Hospital

• CHEDRESE P.

  Department of Obstetrics, Gynecology and Reproductive Sciences, University of Saskatchewan, Royal University Hospital

関連論文

• Cloning of the Elk TSH β-Subunit cDNA and Seasonal Expression of the Pituitary Glycoprotein Hormone Genes
• エルクTSHβ鎖のcDNAクローニングと下垂体糖タンパク質ホルモン遺伝子発現の季節変動
• Cloning of the Elk Common Glycoprotein α-subunit and the FSH and LH β-subunit cDNAs
• 下垂体糖タンパク質ホルモン共通のα鎖と、FSHおよびLHβ鎖の、エルクにおけるcDNAのクローニング

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