単離されたマウス卵核胞の凍結保存

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概要

• 論文の詳細を見る

• The storage of unfertilized oocytes, either immature, maturing or mature, is still unsatisfactory. Here we describe an approach in which germinal vesicles isolated as karyoplasts from immature oocytes are vitrified by open the pulled straws (OPS) method in evacuated porcine zonae pellucidae. After thawing, their survival was almost absolute. Moreover, when thawed GV-karyoplasts were fused to immature oocyte cytoplasts the maturation of reconstructed cells resulted in the production of secondary oocytes - metaphase II.

• 日本繁殖生物学会の論文

著者

• Fulka Josef

  Institute Of Animal Physiology And Genetics

• FULKA Helena

  Institute of Animal Science

• Kren Radomir

  Institute of Animal Production

関連論文

• Intracytoplasmic Sperm Injection in the Pig : Where is the Problem?
• Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes
• 単離されたマウス卵核胞の凍結保存
• Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and In Vivo
• Vitrification of Fully Grown and Growing Porcine Oocytes Using Germinal Vesicle Transfer
• Epigenetic Characteristics of Paternal Chromatin in Interspecies Zygotes
• How to Repair the Oocyte and Zygote?
• Analysis of Marker Expression in Porcine Cell Lines Derived from Blastocysts Produced In Vitro and in Vivo
• Production of Giant Mouse Oocyte Nucleoli and Assessment of Their Protein Content
• Cryopreservation of Isolated Mouse Germinal Vesicles

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