Acrosome Reaction of Mouse Epididymal Sperm on Oocyte Zona Pellucida
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概要
- 論文の詳細を見る
To improve assessment of the acrosome reaction of mouse epididymal sperm, we employed anti-Izumo1 antibody instead of antibodies against acrosomal proteins. The acrosomal states among acrosome-intact, spontaneously acrosome-reacted, truly acrosome-reacted, and probably dead and/or membrane-damaged sperm were clearly distinguished by combined application of anti-Izumo1 antibody, DNA dye Hoechst 33342, and monoclonal antibody MN7 to paraformaldehyde-fixed sperm. When the acrosome reaction of capacitated epididymal sperm on the oocyte zona pellucida was examined using anti-Izumo1 antibody, approximately 20% of sperm bound onto the zona pellucida were acrosome-reacted 30 min after insemination. We also observed the moment of the acrosome reaction of live sperm on the zona pellucida by time-lapse monitoring using fluorescein isothiocyanate-conjugated anti-Izumo1 antibody.
- 日本繁殖生物学会の論文
著者
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Baba Tadashi
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Yamagata Kazuo
Graduate School Of Integrated Science And Art University Of East Asia
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Nakanishi Tomoko
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Yamagata Kazuo
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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YAMASHITA Misuzu
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Tsumura Keiko
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Yamashita Misuzu
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Yamagata Kazuo
Graduate School of Life and Environmental Sciences, University of Tsukuba
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BABA Tadashi
Graduate School of Life and Environmental Science, University of Tsukuba
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