Action of IGF-I on Expression of the Long Form of the Leptin Receptor (ObRb) in the Prepubertal Period and Throughout the Estrous Cycle in the Mature Pig Ovary

スポンサーリンク

概要

• 論文の詳細を見る

• Leptin is an important satiety hormone and reproductive regulator found, along with its receptors, throughout the ovary. To date, the changes in ovarian expression of leptin receptor during the prepubertal period and throughout the estrous cycle in the pig have not been studied. In this study, the long form of the leptin receptor was a detectable level in immature pig ovarian follicles when assayed using semiquantitative reverse transcription-polimerase chain reaction (RT-PCR). Moreover, its level was increased in growing follicles during follicular phase of the estrous cycle (6-fold in early antral [EAF] and antral [AF] follicles) and was highest in newly formed corpora lutea. Its changes paralleled those in steroid secretion and especially progesterone (P4) secretion. Additionally, we showed that IGF-I had stimulatory effect on leptin receptor expression using a tissue culture model of follicular and luteal cells, with a 12-fold increase in prepubertal ovaries and a 1.5- to 2-fold increase in growing follicles and newly formed corpora lutea (CL). These results suggest that ovarian expression of leptin receptor is regulated throughout the estrous cycle by ovarian steroids, with peak expression at ovulation, indicating a possible involvement in follicular development and corpus luteum formation. Moreover, this data points to an important role of IGF-I in leptin receptor expression during the entire estrous cycle, with a special role during the prepubertal period.

• 日本繁殖生物学会の論文

著者

• Nowak Krzysztof

  Department Of Animal Physiology And Biochemistry The August Cieszkowski Agricultural University

• Ptak Anna

  Department Of Physiology And Toxicology Of Reproduction Institute Of Zoology Jagiellonian University

• Ptak Anna

  Laboratory Of Physiology And Toxicology Of Reproduction Department Of Animal Physiology Institute Of

• WOJCIECHOWICZ Tatiana

  Department of Animal Physiology and Biochemistry, The August Cieszkowski Agricultural University

• Gregoraszczuk Ewa

  Laboratory Of Animal Endocrinology & Tissue Culture Department Of Animal Physiology Institute Of

• Wojciechowicz Tatiana

  Department Of Animal Physiology And Biochemistry The August Cieszkowski Agricultural University

• Nowak Krzysztof

  Department Of Animal Physiology And Biochemistry August Cieszkowski University Of Agriculture

関連論文

• in vitroでの豚の成熟および発育段階の黄体におけるアポトーシスとGH,IGF-1誘導プロゲステロン分泌に対するレプチンの効果
• Action of IGF-I on Expression of the Long Form of the Leptin Receptor (ObRb) in the Prepubertal Period and Throughout the Estrous Cycle in the Mature Pig Ovary
• In Vitro Effect of Leptin on Growth Hormone (GH)- and Insulin-Like Growth Factor-I (IGF-I)-stimulated Progesterone Secretion and Apoptosis in Developing and Mature Corpora Lutea of Pig Ovaries
• Action of IGF-I on Expression of the Long Form of the Leptin Receptor (ObRb) in the Prepubertal Period and Throughout the Estrous Cycle in the Mature Pig Ovary
• Valproate Inhibits the Conversion of Testosterone to Estradiol and Acts as an Apoptotic Agent in Growing Porcine Ovarian Follicular Cells
• Prolactin Binding Analysis and Immunohistochemical Localization of Prolactin Receptor in Porcine Ovarian Cells
• Expression of the Beacon Gene in the Rat Pancreatic Islets : Opposite Effects of Beacon (47-73) Protein (Ubiquitin-like Protein 5) on Insulin Secretion In Vivo and Insulin Release by Isolated Islets

スポンサーリンク