Study of Enteral versus Parenteral Application of the Gonadotropin Releasing Hormone Agonist Gonadorelin[6-D-Phe] (D-Phe6-LHRH) on LH Secretion in Goettinger Miniature Pigs

スポンサーリンク

概要

• 論文の詳細を見る

• With respect to the assessment of residue situation and as a part of preclinical trials to determine the biological activities of potential gonadotropin releasing hormone (GnRH) residues in porcine organisms the GnRH agonist Gonadorelin[6-D-Phe] (D-Phesup6/sup-LHRH) was administered either enterally or intramuscularly (i.m.) to female Goettinger miniature pigs in order to evaluate the GnRH-induced luteinizing hormone (LH) surge. Gilts received an (i) enteral application of 10 mg D-Phesup6/sup-LHRH ivia/i a probang (ienteral group/i, n=7), (ii) i.m. injection of 0.1 mg D-Phesup6/sup-LHRH (iparenteral group/i, n=5), or (iii) saline injection (icontrol group/i, n=4). The GnRH and saline applications were repeated every second day with up to seven repetitions. Blood samples were collected ivia/i previously fitted jugular catheters immediately before injections, over an 8 h period in 1 h intervals beginning 2 h after injections, and at 24, 26, 28 and 30 h after applications. Enteral application of D-Phesup6/sup-LHRH induced an LH surge in 23 of 30 treatments. All gilts in the parenteral group exhibited LH release after each D-Phesup6/sup-LHRH application (P0.05), whereas no LH surges were observed after saline injection in the control group. A significant (P0.05) LH rise to mean maximum LH concentrations of 3.25 ± 0.43 and 3.05 ± 0.26 ng/ml occurred in both the enteral and parenteral groups, but there was no difference in the time interval after GnRH (2.6 ± 0.3 vs. 2.3 ± 0.3 h) and the mean duration of LH peak (6.5 ± 0.4 and 6.8 ± 0.3 h) between the treatment groups. In conclusion, (i) enteral application of 10 mg D-Phesup6/sup-LHRH induced LH release in a physiological range from the pituitary of female minipigs, and (ii) neither an accumulative effect nor a cumulative LH response were found after repeated GnRH application. Furthermore, (iii) in regard to consumer protection and gonadotropin secretion, D-Phesup6/sup-LHRH residues can be excluded from having long-term effects.br

著者

• Brussow Klaus-peter

  Department Of Reproductive Biology Fbn Research Institute For The Biology Of Farm Animals

• KANITZ Ellen

  Department of Behavioural Physiolog, FBN Research Institute for the Biology of Farm Animals

• TUCHSCHERER Armin

  Department of Genetic and Biometry, FBN Research Institute for the Biology of Farm Animals

• TOSCH Petra

  VetCom-pharma GmbH

関連論文

• Follicular Size is Associated with the Levels of Transcripts and Proteins of Selected Molecules Responsible for the Fertilization Ability of Oocytes of Puberal Gilts
• 副腎皮質刺激ホルモンの反復投与によって妊娠ブタの生殖ホルモンレベルが変動し、それは出生前後期の仔ブタのホルモン分泌に影響する
• 副腎皮質刺激ホルモンの反復投与によって妊娠ブタの生殖ホルモンレベルが変動し、それは出生前後期の仔ブタのホルモン分泌に影響する
• Study of Enteral versus Parenteral Application of the Gonadotropin Releasing Hormone Agonist Gonadorelin[6-D-Phe] (D-Phe6-LHRH) on LH Secretion in Goettinger Miniature Pigs

スポンサーリンク