Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods
スポンサーリンク
概要
- 論文の詳細を見る
Bacteria in the rhizosphere influence plant growth and interact with plant roots. Microscopy and culture method have been used for studies of microorganisms of the rhizosphere, but these methods are insufficient for evaluation because most rhizosphere bacteria are viable but non-culturable (VBNC). Bacteria in the rhizosphere of rice cultivated in Andosol lowland and upland fields were analyzed in this study using PCR-DGGE and FISH, in combination with modified pretreatments. Results show that the two methods with the pretreatments, more than conventional methods, provided a rapid and simple analysis of rhizosphere bacteria. The 16S rDNA band pattern of bacteria in the rhizosphere obtained using PCR-DGGE indicated different species composition of bacterial community in the two ecosystems and greater diversity of bacteria in the rhizosphere in upland field. Sequencing of major 16S rDNA bands identified Bacterium A35 and Clostridium bifermentans as dominant bacteria in the rhizosphere of rice in lowland fields and Klebsiella planticola and Bacillus fusiformis in upland fields. Furthermore, FISH observation indicated the predominance of gram-positive low GC bacteria in both rhizospheres and a higher proportion of Clostridium spp. in lowland fields, which is consistent with results of PCR-DGGE analysis. The results suggest that the bacteria in the rice rhizosphere can be changed depending on aerobic and anaerobic conditions of fields. It is expected to apply the PCR-DGGE and FISH to agricultural field experiments as reliable methods to evaluate the rhizosphere bacteria.
- Japanese Society for Root Research,根研究会,Field Production Science Center, Graduate School of Agriculture and Life Sciences, The University of Tokyo,Faculty of Agriculture, The University of Tokyo,AE-Bio, Graduate School of Agriculture and Life Sciences, Thの論文
著者
-
Morita Shigenori
Field Production Sci. Center Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
-
Abe Jun
Ae-bio Graduate School Of Agricultural And Life Sciences The Univ. Of Tokyo
-
Doi Tetsuya
Field Production Science Center, Graduate School of Agriculture and Life Sciences, The University of
-
Hagiwara Yusuke
Faculty of Agriculture, The University of Tokyo.
-
Abe Jun
AE-Bio, Graduate School of Agriculture and Life Sciences, The University of Tokyo.
-
Morita Shigenori
Field Production Science Center, Graduate School of Agriculture and Life Sciences, The University of Tokyo.
-
Doi Tetsuya
Field Production Science Center, Graduate School of Agriculture and Life Sciences, The University of Tokyo.
関連論文
- Diurnal and Phenological Changes in the Rate of Nitrogen Transportation Monitored by Bleeding in Field-Grown Rice Plants (Oryza sativa L.)(Crop Physiology and Ecology)
- Study on rhizosphere bacterial community in lowland rice grown with organic fertilizers by using PCR-denaturing gradient gel electrophoresis
- Root Anatomical Responses to Waterlogging at Seedling Stage of Three Cordage Fiber Crops(Crop Morphology)
- Structure and Function of Rice Root System under FACE Condition
- Nitrogen-Fixing Activity of Root Nodules in Relation to Their Size in Peanut (Arachis hypogaea L.)(Crop Physiology and Ecology)
- Distribution Pattern of Root Nodules in Relation to Root Architecture in Two Leading Cultivars of Peanut (Arachis hypogaea L.) in Japan(Crop Morphology)
- Root cortex : structural and functional variability and responses to environmental stress
- Variation in root morphology and anatomy among accessions of cultivated rice (Oryza sativa L.) with different genetic backgrounds
- Evaluation of Water-Saving Rice-Winter Crop Rotation System in a Suburb of Tokyo(Agronomy)
- The use of berberin and fluorol yellow for confocal laser scanning microscopy of plant roots
- Silicon deposition in roots and leaves varies among forage grasses
- ESTABLISHMENT OF A RAPID METHOD TO ANALYZE CELL ELONGATION IN DIFFERENT TISSUES ALONG ROOT AXIS IN MAIZE
- Development and Distribution of Root System in Two Grain Sorghum Cultivars Originated from Sudan under Drought Stress(Crop Physiology and Ecology)
- Identification of Casparian Bands in the Mesocotyl and Lower Internodes of Rice (Oryza sativa L.) Seedlings Using Fluorescence Microscopy(Crop Morphology)
- Analysis of rhizosphere bacteria of rice cultivated in Andosol lowland and upland fields using molecular biological methods
- Varietal Differences in Stem Diameter and Rooting Number of Phytomers in Conjunction with Root System Development of Field-Grown Rice (Oryza sativa L.)(Crop Morphology)
- Growth of Rice (Oryza sativa L.) Cultivars under Upland Conditions with Different Levels of Water Supply : 3. Root System Development, Soil Moisture Change and Plant Water Status(Crop Physiology and Ecology)
- Growth of Three Rice (Oryza sativa L.) Cultivars under Upland Conditions with Different Levels of Water Supply 1. Nitrogen Content and Dry Matter Production(Agronomy)
- Seasonal changes of starch grain accumulation in roots of Erianthus
- The necessity of root research in Asia