Enhanced Expression of Apoptin by the Myc-Max Binding Motif and SV40 Enhancer for SCLC Gene Therapy

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概要

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• Apoptin is derived from chicken anemia virus (CAV) and known to induce tumor specific apoptosis but not normal cells. The aim of this study was to use increased expression of apoptin by the Myc–Max response element (MMRE) and SV40 enhancer in small-cell lung cancer (SCLC) gene therapy. To investigate the possibility of the utilization of the MMRE, apoptin, and SV40 promoter/enhancer in targeted cancer gene therapy, adenovirus vector expressing apoptin controlled by the MMRE, and SV40 promoter/enhancer was constructed. Ad-MMRE-apoptin-enh infected SCLC cells were significantly suppressed and induced apoptosis more than those of Ad-apoptin or Ad-apoptin-enh. Infection with Ad-MMRE-apoptin-enh of normal cells did not increase apoptosis. About 85% of SCLC tumors show overexpression of the myc family, so the increased expression of apoptin by MMRE and SV40 enhancer can be used in targeted SCLC gene therapy. These results indicate that apoptin expression was increased by the MMRE and SV40 promoter/enhancer, and that this strategy can be used in SCLC targeted cancer gene therapy.

• 社団法人 日本農芸化学会の論文
• 2005-01-23

著者

• Song Joon-seok

  Institute Of Biotechnology Korea University

関連論文

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• Activity of the Human Telomerase Catalytic Subunit (hTERT) Gene Promoter Could Be Increased by the SV40 Enhancer
• Adenovirus-mediated Suicide Gene Therapy Using the Human Telomerase Catalytic Subunit (hTERT) Gene Promoter Induced Apoptosis of Ovarian Cancer Cell Line(Biochemistry & Molecular Biology)
• Enhanced Expression of Apoptin by the Myc-Max Binding Motif and SV40 Enhancer for SCLC Gene Therapy

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