Single Microspore Reverse Transcription-Polymerase Chain Reaction : Dynamic Change of Gene Expression during Microsporogenesis
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概要
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A sensitive and reliable single microspore reverse transcription-polymerase chain reaction (RT-PCR) assay was developed that requires little sample material and only a short sampling time and ensures template preservation. We propose that the assay provides a useful tool to study not only cell-cycle related events in developing pollen but also the molecular mechanisms underlying male sterility and mutants impaired in pollen development.
- 社団法人 日本農芸化学会の論文
- 2005-11-23
著者
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Zhou Bing
Key Laboratory Of Moe For Plant Developmental Biology College Of Life Science Wuhan University
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Sauter Margret
Botanisches Institut Universitaet Kiel
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SUN Mengxiang
Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University
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Sun Mengxiang
Key Laboratory Of Moe For Plant Developmental Biology College Of Life Science Wuhan University