Microbial Production of L-Ascorbic Acid from D-Sorbitol, L-Sorbose, L-Gulose, and L-Sorbosone by Ketogulonicigenium vulgare DSM 4025

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概要

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• Ketogulonicigenium vulgare DSM 4025, known as a 2-keto-L-gulonic acid producing strain from L-sorbose via L-sorbosone, surprisingly produced L-ascorbic acid from D-sorbitol, L-sorbose, L-gulose, and L-sorbosone as the substrate under a growing or resting condition. As the best result, K. vulgare DSM 4025 produced 1.37 g per liter of L-AA from 5.00 g per liter of L-sorbosone during 4 h incubation time at 30 °C under the resting cell condition having 5.70 g per liter of wet cells. The precursor of L-AA formation from D-sorbitol and L-sorbose, except for L-gulose, was thought to be the putative furanose form of L-sorbosone. This is the first time it is reported that bacteria can produce vitamin C via L-sorbosone.

• 2005-03-23

著者

• Hoshino Tatsuo

  Tamagawa University Research Institute

• MIYAZAKI Taro

  Department of Applied Microbiology, Nippon Roche Research Center

• Sugisawa Teruhide

  Biotechnology R&d Dsm Nutritional Products Ltd.

• Miyazaki Taro

  Department Of Genome Antibody Product Research Chugai Pharmaceutical Co. Ltd. Fuji Gotemba Research

関連論文

• Microbial Production of L-Ascorbic Acid from D-Sorbitol, L-Sorbose, L-Gulose, and L-Sorbosone by Ketogulonicigenium vulgare DSM 4025
• Main Polyol Dehydrogenase of Gluconobacter suboxydans IFO 3255, Membrane-bound D-Sorbitol Dehydrogenase, That Needs Product of Upstream Gene, sldB, for Activity(Biochemistry & Molecular Biology)
• Molecular cloning and functional expression of D-sorbitol dehydrogenase from Gluconobacter suboxydans IFO3255, which requires pyrroloquinoline quinone and hydrophobic protein SldB for activity development in E.coli(Biochemistry & Molecular Biology)

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