Quantification of Indole-3-Acetic Acid and Amino Acid Conjugates in Rice by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry
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概要
- 論文の詳細を見る
A method for quantifying indole-3-acetic acid (IAA) and its conjugates with the six amino acids, Ala, -Asp, -Ile, -Glu, -Phe and -Val, in rice (Oryza sativa) by using high-performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (HPLC–ESI–MS/MS) is described. Samples from the rice plant or callus were treated with 80% acetone in water containing 2.5 mM diethyl dithiocarbamate. Each extract was partially purified in C18 cartridge column for solid-phase extraction (SPE) and subjected to HPLC–ESI–MS/MS without converting the product. The detection limit was 3.8 fmol for IAA, and 0.4–2.9 fmol for the IAA amino acid conjugates. The method was applied to the analysis of IAA and its conjugates in rice seedlings, dehulled rice and calli, using 20–100 mg tissue samples.
- 社団法人 日本農芸化学会の論文
- 2005-04-23
著者
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MATSUDA Fumio
Plant Functions and Their Control, CREST, Japan Science and Technology Agency
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MIYAZAWA Haruna
Plant Functions and Their Control, CREST, Japan Science and Technology Agency
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WAKASA Kyo
Plant Functions and Their Control, CREST, Japan Science and Technology Agency
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MIYAGAWA Hisashi
Plant Functions and Their Control, CREST, Japan Science and Technology Agency
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Wakasa Kyo
Plant Functions And Their Control Crest Japan Science And Technology Agency
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Wakasa Kyo
Biotech-breeding Lab. National Agriculture Research Center
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Matsuda Fumio
Plant Functions And Their Control Crest Japan Science And Technology Agency
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Miyagawa Hisashi
Plant Functions And Their Control Crest Japan Science And Technology Agency
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Miyagawa Hisashi
Department Of Agricultural Chemistry Kyoto University
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Hisashi Miyagawa
Division Of Applied Life Sciences Graduate School Of Agriculture Kyoto University
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Miyagawa Hisashi
Kyoto Univ. Kyoto Jpn
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