A Variant Protein from φ29 Replication Gene, Gene 1, Did Not Form Homo-Polymer Due to a Single Amino Acid Substitution Near the Carboxyl Terminus

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概要

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• For study of the self-association of the product of φ29 gene 1, one variant which has a substitution at the 71st amino acid was used. By glycerol gradient sedimentation, the product of wild-type gene 1 existed both as large aggregate and oligomer, whereas the variant was detected as a single peak of monomer size. According to experiments using His-tagged proteins and Ni-NTA magnetic beads, the variant made only a little self-associated complex. From these results, a site essential for self-association was suggested to exist close to the carboxyl terminus of the product of φ29 gene 1.

• 社団法人 日本農芸化学会の論文
• 2005-05-23

著者

• Makino Osamu

  Department Of Genetics Life Science Institute Sophia University

• TAKEUCHI Ari

  Department of Genetics, Life Science Institute, Sophia University

• HASHIYAMA Kazuya

  Department of Genetics, Life Science Institute, Sophia University

• Hashiyama Kazuya

  Department Of Genetics Life Science Institute Sophia University

• Takeuchi Ari

  Department Of Genetics Life Science Institute Sophia University

関連論文

• Isolation of a series of single missense mutants of a dna gene of phage φ29, gene 1, utilizing their inhibitory effect on E. coli growth
• A Variant Protein from φ29 Replication Gene, Gene 1, Did Not Form Homo-Polymer Due to a Single Amino Acid Substitution Near the Carboxyl Terminus
• Stereochemical Studies on the Nuclear Alkylation of Phenols with 1-Phenylethyl Chloride and the Acidic Rearrangement of the 1-Phenylethyl Ethers of Phenols in the Phenolic Solvents

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