Identification, Cloning, and Expression of the Scytalidium acidophilum XYL1 Gene Encoding for an Acidophilic Xylanase
スポンサーリンク
概要
- 論文の詳細を見る
We cloned XYL1, a Scytalidium acidophilum gene encoding for an acidophilic family 11 xylanase. The XYL1p protein was expressed in Pichia pastoris using the pPICZαA expression plasmid. The secreted protein was purified by TAXI affinity column chromatography. The purified XYL1p showed an optimum activity at pH 3.2 and 56 °C. The Michaelis–Menten constants were determined.
- 社団法人 日本農芸化学会の論文
- 2006-01-23
著者
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Wouters Johan
Biological Structural Chemistry Laboratory University Of Namur
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Rossini Carlos
Research Unit In Molecular Biology University Of Namur
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BALAA Bassam
Research Unit in Molecular Biology, University of Namur
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DOGNE Sophie
Research Unit in Molecular Biology, University of Namur
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SCHAUS Jean-Michel
Research Unit in Molecular Biology, University of Namur
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DEPIEREUX Eric
Research Unit in Molecular Biology, University of Namur
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VANDENHAUTE Jean
Research Unit in Molecular Biology, University of Namur
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HOUSEN Isabelle
Research Unit in Molecular Biology, University of Namur
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Dogne Sophie
Research Unit In Molecular Biology University Of Namur
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Balaa Bassam
Research Unit In Molecular Biology University Of Namur
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Depiereux Eric
Research Unit In Molecular Biology University Of Namur
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Vandenhaute Jean
Research Unit In Molecular Biology University Of Namur
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Schaus Jean-michel
Research Unit In Molecular Biology University Of Namur
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Housen Isabelle
Research Unit In Molecular Biology University Of Namur
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AL BALAA
Research Unit in Molecular Biology, University of Namur