Ester Formation by Alcohol Acetyltransferase from Brewers Yeast

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概要

• 論文の詳細を見る

• Alcohol acetyltransferase responsible for the formation of acetate esters during beer fermentation was found to be localized at the cell membrane of brewers yeast. This cell membrane-bound enzyme was purified 120-fold by solubilization with Triton X-100, gel filtration on a Sepharose 6B column and chromatography on a DEAE-Sephadex A-50 column. The enzyme was most active at 30°C at pH 78. It was least active against C3 alcohol among C1C6 alcohols, and slightly more active against straight-chain alcohols than against branched-chain alcohols with the same carbon number. The enzyme was strongly inhibited by unsaturated fatty acids, heavy metal ions and sulfhydryl reagents.

• 社団法人 日本農芸化学会の論文

著者

• HASHIMOTO Naoki

  The Research Laboratories of Kirin Brewery Co.

• YOSHIOKA Kazuo

  The Research Laboratories of Kirin Brewery Co., Ltd.

関連論文

• A New Micromethod for Determination of Acetyl-CoA with Alcohol Acetyltransferase
• Ester Formation by Alcohol Acetyltransferase from Brewers Yeast

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