Studies on regeneration media for Bacillus subtilis protoplasts.
スポンサーリンク
概要
- 論文の詳細を見る
Bacillus subtilis protoplasts regenerate on media containing horse serum, bovine serum or gelatin. These compounds could be replaced by polyvinyl pyrrolidone or dextran, and a medium which contained 30 g polyvinyl pyrrolidone and 20 mg casamino acids per liter with chemically defined ingredients was especially useful for selection of prototrophs, e.g., by protoplast fusion. Polyvinyl pyrrolidone and other plasma expanders stimulated protoplast division in liquid media and improved protoplast survival on agar media.
- 公益社団法人 日本農芸化学会の論文