Application of an immobilized Escherichia coli cell tube in analysis of L-threonine.

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概要

• 論文の詳細を見る

• Acetone-dried cells of Escherichia coli with an enhanced level of biodegradative L-threonine deaminase (EC 4.2.1.16) were easily and tightly immobilized to the inner surface of glass tubes by use of an emulsion-type of photo-crosslinkable resin prepolymer. A continuous flow system equipped with the microbial cell tube thus prepared was proved to be applicable for assay of L-threonine or L-serine in fermentation broth with rapidity (each assay, less than 9min), excellent substrate specificity (specific for L-threonine and L-serine) and high stability over repeated assays (at least 60 assays).

• 公益社団法人 日本農芸化学会の論文

著者

• WATANABE Kunihiko

  Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University

関連論文

• Evaluation of the Recognition Ability of Molecularly Imprinted Proteins by Surface Plasmon Resonance (SPR) Spectroscopy
• Dextran-bound AMP as the Allosteric Effector for Entrapped Threonine Deaminase
• Application of an immobilized Escherichia coli cell tube in analysis of L-threonine.

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