Transformation of bacillus protoplasts by plasmid pTP4 DNA.

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概要

• 論文の詳細を見る

• Polyethylene glycol (PEG)-induced protoplast transformation by plasmid pTP4 DNA encoded chloramphenicol resistance determinant was developed for Bacillus subtilis, B. amyloliquefaciens, B. licheniformis, B. megaterium and B. pumilus. Protoplasts were formed by treatment of cells with lysozyme and the transformation frequencies (transformants per regenerants) were in the range of 1.3×10-2 to 7.1×10-1. Reisolated plasmid DNA prepared from transformants exhibited covalently closed and open circular forms similar to those of the donor DNA. These results indicate that PEG-induced protoplast transformation is an adequate method for plasmid transformation and pTP4 is a useful plasmid as a cloning vector in a wide range of varieties of the genus Bacillus.

• 公益社団法人 日本農芸化学会の論文

著者

• SEKIGUCHI Junichi

  Department of Applied Microbial Technology, The Kumamoto Institute of Technology

関連論文

• Transformation of bacillus protoplasts by plasmid pTP4 DNA.

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