On the Formation of a Conjugate of PCBs with Glutathione and Its Further Metabolism in Mice or Rats

概要

論文の詳細を見る
In in vivo and in vitro experiments using mice or rats, an N-acetyl-cysteine derivative, so-called mercapturic acid, was demonstrated with PCBs by gas chromatography-mass spectrometry with, use of the sample after methylation and/or acetylation of their metabolites. The derivative was suggested from its chromatographic pattern to be derived from the conjugate of PCBs with glutathione, although direct evidence has not been obtained as yet, owing to the extreme difficulty in purifying this conjugate on account of its characteristic of being soluble only in water, and to the limited capacity of the apparatus for mass spectrometric analysis. And yet, in the experiment using chlorobenzene an N-acetyl-cysteinylglycine derivative was found, whichcould further support the possibility of conjugate formation of PCBs, because the former was observed to behave quite similarly to the latter. On the other hand, it was recognized in urine that these conjugates could further be metabolized into an acetic acid derivative via pyruvate without only mercapturic acid as an end metabolite.
社団法人日本農芸化学会の論文