Purification of Four Esterases from Aspergillus niger NRRL 337

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概要

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• Aspergillus rager NRRL 337, a significant lipase producer, also produced esterases in a semisolid medium. From the culture extract, four esterases (Esterase-I, -II, -III, -IV) were purified by means of ion-exchange chromatography, gel nitration, gel permeation with high performance liquid chromatography, and preparative disc gel electrophoresis. Homogeneity of each esterase was confirmed using disc gel electrophoresis. The four esterases were found to exhibit low isoelectric points (pH 3.63.9). The molecular weights determined by gel permeation chromatography of Esterase-I, Esterase-II, Esterase-III and Esterase-IV were 23000, 127000, 29500 and 27000, respectively.

• 社団法人 日本農芸化学会の論文

著者

• OKUMURA Susumu

  Osaka Municipal Technical Research Institute

• IWAI Mieko

  Osaka Municipal Technical Research Institute

• LEAL Elizabeth

  National University of Colombia

• TSUJISAKA Yoshio

  Osaka Municipal Technical Research Institute

関連論文

• Synthesis of Ester Oligomer by Aspergillus niger Lipase
• Properties and Substrate Specificities of Four Esterases from Aspergillus niger NRRL 337
• The Effect of Reverse Action on Triglyceride Hydrolysis by Lipase
• Purification of Four Esterases from Aspergillus niger NRRL 337

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