Development of an Oxygen-resistant Hydrogen Bacterium during Plate Cultivation under High Oxygen Tension

概要

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An oxygen-resistant hydrogen bacterium developed during an autotrophic plate culture under 40%O2. Whenoxygen-sensitive strain N34 was incubated under an atmosphere of 50%H2 +40% O2+10% CO2, small white colonies (SW) first formed, and then, large yellow colonies (LY) developed from some of the SW. The LYcounts increased gradually. The hydrogenase activity of SWwas one-fifth that of LY. The growth lag period of a liquid culture under 40% O2 inoculated with SWwas more than 100 hr, while it was almost zero with LY, indicating that SWwere oxygensensitive and LYoxygen-resistant. Whena single SWwas transferred to a fresh plate culture under 40% O2 at first SWand then LY were formed again. On the other hand, LYwere always formed under 40%O2 when a single LYwas transferred. From these data, it was concluded that SWwere formed from oxygen-sensitive strain N34 whose growth was inhibited under 40%O2 and that oxygen-resistant LYwere formed as a result of adaptation of SWto high oxygen tension.
社団法人日本農芸化学会の論文