Isolation and characterization of a temperature-sensitive sporulation mutant of Bacillus subtilis.

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概要

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• Temperature-sensitive sporulation mutants were isolated spontaneously from Bacillus subtilis 168 TT by a sequential transfer method. A representative mutant strain, ts32, was characterized in detail. The mutant grew normally at 30°C and 42°C, but did not sporulate at 42°C. Electron microscopic observation and physiological analysis showed that the mutant was blocked at stage 0-1 ofsporulation. Genetic analysis suggested that the mutation was located at the spo0B locus on the B. subtilis chromosome. Temperature-shift experiments clearly showed that the spo0B gene product functions only at the beginning of sporulation.

• 公益社団法人 日本農芸化学会の論文

著者

• SATO Kazuki

  Department of Biology, Faculty of Science, Hiroshima University

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