Photomodification by Rose Bengal of NAD(P)H-Nitrate Reductase of Hansenula anomala
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概要
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The photoillumination of assimilatory nitrate reductase of Hansenula anomala in the presence of Rose Bengal caused a selective inactivation of NAD(P)H-linked activities. The potent protective effect of NAD(P)H suggests that the residue(s) modified by the treatment is involved in electron transport from NAD(P)H. From the observation that p-chloromercuribenzoate-treated enzyme was potomodified similarly to the native enzyme, the photomodified residue(s) is deduced not to be cysteine. The parallel decrease of NAD(P)H-nitrate reductase activity and the histidine contents suggests that histidine residue(s) is involved in this inactivation.
- 社団法人 日本農芸化学会の論文
著者
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Minagawa Nobuko
Department Of Biochemistry Niigata College Of Pharmacy
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Yoshimoto Akio
Department Of Biochemistry Niigata College Of Pharmacy
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