Biosynthesis of L-lysine and L-threonine in Brevibacterium. Part IX. Production of L-lysine by fluoropyruvate-sensitive mutants of Brevibacterium lactofermentum.

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概要

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• Better producers of L-lysine were obtained by derivation of fluoropyruvate(FP)-sensitive mutants from Brevibacterium lactofermentum AJ3990. The coexistence of FP and excess biotin synergistically stimulated L-lysine formation by washed cells. FP inhibited 50% of growth and pyruvate dehydrogenase (PDH) activity of AJ3990 at 0.04 HIM and ImM, respectively. Therefore, the synergistic effect of FP and excess biotin seems to be due to the optimization of the PDH/pyruvate carboxylase activity ratio in L-lysine biosynthesis. This was confirmed by the derivation of FP-sensitive mutants which have the optimal level of PDH activity for L-lysine production. The best producer, AJ11204, had about 27% PDH activity as compared with the parental strain and accumulated 70 g of L-lysine per liter with a conversion yield of 50% from glucose in the presence of excess biotin.

• 公益社団法人 日本農芸化学会の論文

著者

• TAKINAMI Koichi

  Central Research Laboratories of Ajinomoto Co.

• YOSHIHARA Yasuhiko

  Central Research Laboratories of Ajinomoto Co.

関連論文

• N^ε-Acyllysine Derivatives Inhibit Aspartokinase in Brevibacterium lactofermentum
• Selection and Breeding of Lysine-accumulating Saccharomyces cerevisiae as a Stable Source of Lysine in the Rumen
• Fermentative Production of L-Glutamine by Sulfaguanidine Resistant Mutants Derived from L-Glutamate Producing Bacteria
• Biosynthesis of L-lysine and L-threonine in Brevibacterium. Part IX. Production of L-lysine by fluoropyruvate-sensitive mutants of Brevibacterium lactofermentum.

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