Ag+-Sensitive NADH Dehydrogenase in the Na+-Motive Respiratory Chain of the Marine Bacterium Vibrio alginolyticus

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概要

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• Components of the Na+-motive NADH: quinone oxidoreductase segment in the respiratory chain of Vibrio alginolyticus were examined in membranes prepared from wild type, Na+-pump-defective mutants, and a spontaneous revertant. Ag+ distinguished two kinds of respiratory NADH dehydrogenases. The Na+-pump-defective mutants lacked Ag+-sensitive NADH dehydrogenase activity. Incubation of the Ag+-sensitive NADH dehydrogenase with solubilized membrane proteins of the mutant led to the reconstitution of Na+-motive NADH: quinone oxidoreductase activity. We think that Ag+-sensitive NADH dehydrogenase is an essential component of the respiratory Na+ pump of this organism.

• 社団法人 日本農芸化学会の論文

著者

• Asano Makoto

  Department Of Anesthesiology Sapporo Medical College

• HAYASHI Maki

  Department of Enzymology, Research Institute for Chemobiodynamics, Chiba University

• TOKUDA Hajime

  Department of Membrane Biochemistry, Chiba University

• UNEMOTO Tsutomu

  Department of Membrane Biochemistry, Chiba University

• ASANO Makoto

  Department of Membrane Biochemistry, Chiba University

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• Ag+-Sensitive NADH Dehydrogenase in the Na+-Motive Respiratory Chain of the Marine Bacterium Vibrio alginolyticus

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