Purification and Some Properties of Isocitrate Lyase from the Pollen of Pinus densiflora Sieb. et Zucc.

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概要

• 論文の詳細を見る

• Isocitrate lyase (EC 4.1.3.1) was purified from the pollen of Pinus densiflora Sieb. et Zucc. to apparent homogeneity as judged by SDS-PAGE. The molecular weight of the enzyme was 200, 000 by glycerol density gradient analysis and gel filtration on Sepharose 6B, and the subunit molecular weight was 65, 000 by SDS-PAGE. The enzyme was maximumally active at pH 7.6 in the presence of 3 mM MgCl2 and 0.5 mM EDTA. The enzyme was completely inactivated by heating at 50°C for 5 min, but no activity was lost by the same treatment in the presence-of 3 mM Mg2+ and 0.5 mM EDTA. The enzyme was inhibited by PCMB, and the inhibition was reversed by the addition of an equimolar amount of cysteine. ATP, ADP, 3-phosphoglycerate, phosphoenolpyruvate, tartrate, maleate, oxalate, and itaconate inhibited the enzyme. The Km value for DL-isocitrate was 6.6 × 10-4 M. Itaconate was an uncompetitive inhibitor with respect to isocitrate, and the Ki value for itaconate was 2.8 × 10-6 M.

• 社団法人 日本農芸化学会の論文

著者

• TSUKAMOTO Chigen

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Iwate University

• EJIRI Shin-ichiro

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Iwate University

• KATSUMATA Teizo

  Laboratory of Biochemistry, Department of Agricultural Chemistry, Iwate University

関連論文

• Chemotaxonomic Markers in Brassica Seeds at the Species and Subspecies Levels
• Purification and Some Properties of Isocitrate Lyase from the Pollen of Pinus densiflora Sieb. et Zucc.
• Purification and Some Properties of Malate Synthase from the Pollen of Pinus densiflora Sieb. et Zucc.
• Effects of Dibutyryl Cyclic AMP and Dibutyryl Cyclic GMP on the Changes in Invertase, Lipase, and Some Dehydrogenase Activities during Germination of Pine Pollen

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