Synthesis of enzymatically active mouse submandibular gland renin in Escherichia coli.

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概要

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• A plasmid (pMR226) containing the cDNA coding for the complete amino acid sequence of mouse renin was constructed. The cDNA was reconstructed so as to code for either fused mature renin or preprorenin, and inserted into a plasmid designed to express a protein under the control of the Escherichia coli tryptophan promoter. When introduced into E. coli, the resultant plasmids (pMR304, pMR324) directed the synthesis of proteins that cross-reacted with an antiserum raised against purified mouse renin. The renin precursor produced was readily activated on trypsin treatment and the activity was inhibited by pepstatin and antirenin-antibody.

• 公益社団法人 日本農芸化学会の論文

著者

• NAKANO Eiichi

  Bioscience Research Laboratory, Kikkoman Corporation

関連論文

• Synthesis of enzymatically active mouse submandibular gland renin in Escherichia coli.

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