A novel proteinase inhibitor, tyrostatin, inhibiting some pepstatin-insensitive carboxyl proteinases.

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概要

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• Tyrostatin, a new carboxyl proteinase inhibitor, was discovered in a culture filtrate of an actinomycete. From taxonomical studies, the strain was identified and named Kitasatosporia sp. No. 55. The inhibitor was extracted with ethylacetate, purified by Sephadex LH-20 and silica gel column chromatographies, and crystallized from methanol. The yield was about 100 mg from 18.2 1 of culture filtrate. The structure of tyrostatin was N-isovaleryl-tyrosyl-leucyl-tyrosinal. Tyrostatin strongly inhibited pepstatin-insensitive carboxyl proteinases originating from Pseudomonas sp. No. 101 and Xanthomonas sp. No. T-22, but not any of the other pepstatininsensitive carboxyl proteinases tested. It inhibited all of the carboxyl proteinases tested of the pepstatin-sensitive group, although the inhibitory activities were not so potent. It also strongly inhibited such cysteine proteinases as papain.

• 公益社団法人 日本農芸化学会の論文

著者

• KAINOSHO Masatsune

  Department of chemistry, Faculty of Science, Tokyo Metropolitan University

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• A novel proteinase inhibitor, tyrostatin, inhibiting some pepstatin-insensitive carboxyl proteinases.

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