Embryogenesis and Expression Profiles of charon and Nodal-Pathway Genes in Sinistral (Paralichthys olivaceus) and Dextral (Verasper variegatus) Flounders
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概要
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Although it is well known that flounder form external asymmetry by migration of one eye at metamorphosis, the control system that forms this asymmetry is unknown. To help elucidate this mechanism, we here describe the embryogenesis and expression profiles of the Nodal-pathway genes in the Japanese flounder, Paralichthys olivaceus. We also perform a comparative study of the laterality of the expression of these genes in sinistral (P. olivaceus) and dextral (Verasper variegatus) flounders. In P. olivaceus, Kupffers vesicle forms at the 2-somite stage, after which left-sided expression of spaw starts at the 8-somite stage. Left-sided expression of pitx2 occurs in the gut field at the 15-somite to high-pec stages, in the heart field at the 21-somite stage, and in the dorsal diencephalon at the 27-somite to high-pec stages. In response to left-sided pitx2 expression, the heart, gut, and diencephalon begin asymmetric organogenesis at the pharyngula (heart) and the long-pec (gut and diencephalon) stages, whereas the eyes do not show signs of asymmetry at these stages. In both sinistral and dextral flounders, the Nodal-pathway genes are expressed at the left side of the dorsal diencephalon and left lateral-plate mesoderm. Considering these data together with our previous finding that reversal of eye laterality occurs to some extent in the P. olivaceus mutant reversed, in which embryonic pitx2 expression is randomized, we propose that although the Nodal pathway seems to function to fix eye laterality, embryonic expression of these genes does not act as a direct positional cue for eye laterality.
著者
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HASHIMOTO HISASHI
Bioscience and Biotechnology Center, Nagoya University
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Aritaki Masato
Miyako Station National Center For Stock Enhancement Fisheries Research Agency
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SUZUKI Tohru
Laboratory of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ
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KUROKAWA Tadahide
National Research Institute of Aquaculture
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Hashimoto Hisashi
Laboratory Of Freshwater Fish Stocks Bioscience And Biotechnology Center Nagoya University
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Hashimoto Hisashi
National Research Institute Of Aquaculture Fisheries Research Agency
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Hashimoto Hisashi
Bioscience And Biotechnology Center Nagoya University
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Uji Susumu
National Research Institute of Aquaculture, Fisheries Research Agency
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Aritaki Masato
Miyako Station Japan Sea-farming Association
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Uji Susumu
National Research Institute Of Aquaculture Fisheries Research Agency
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Uozumi Kaori
Hyogo Prefectural Fisheries Experimental Station
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Aritaki M
Seikai National Research Institute Fisheries Research Agency
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Uji Susumu
National Research Institute Of Aquaculture Farming Biology Division Fisheries Research Agency (fra)
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Suzuki Tohru
Laboratory of Bioindustrial Informatics, Graduate School of Agricultural Science, Tohoku University
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Aritaki Masato
Miyako Station, National Center for Stock Enhancement, Fisheries Research Agency
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