抗ウイルス鶏卵抗体（IgY）による受動免疫効果に関する研究-II : キンギョにおける抗GFHNV-IgYの受動免疫効果

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Herpesviral hematopoietic necrosis of goldfish has caused mass mortalities in the USA, Australia and European countries as well as in Japan since 1993. Still we have no ways to treat this disease. We tried passive immunization by intramuscular injections with anti GFHNV-IgY（egg yolk antibody）in order to protect goldfish from GFHNV infection. Injected IgY containing anti GFHNV-IgY was absorbed into the blood serum at high concentration in the day 1 after three injections, which was maintained to the day 7. The immunized fish were challenged by immersion with GFHNV at 10－3 and 10－4 dilution in the day 1 post immunization and were protected from GFHNV infection at 40.0％ and 87.5％ RPS respectively. Histopathological observation revealed no signs of virus infection to hematopoietic cells in survivors, indicating neutralization of virus with IgY. Electron microscopy revealed apoptosis-like features of virus-infected cells, resulting in prevention of viral maturation and virion release out of the cell.
三重大学大学院生物資源学研究科の論文