コイにおけるEdwardsiella tardaに対する免疫反応-I : 不活化ワクチンと生菌ワクチンによる能動免疫効果

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We performed active immunization by intramuscular injections with formalin-killed bacterin and live bacteria of E.tarda in koi Cyprinus carpio, and examined the specific antibody titer and the effectiveness by challenge with virulent bacteria. The mean titer of serum antibody was 214 in koi (n=5) vaccinated by bacterin, and 512 in koi (n=5) vaccinated by live bacteria with low virulence, indicating the successful immunization. After challenge with virulent bacteria, survival ratio was 32% in koi (n=19) vaccinated with bacterin whereas 70% in the control (n=20), resulted in no effectiveness. On the other hand survival ratio was 94% in koi (n=16) vaccinated with live bacteria whereas 27% in the control (n=15), resulted in definite effectiveness of immunization (RPS=91.8%). These results determined that the vaccination using live bacteria with low-virulence induced the definitely effective immunization in koi.
三重大学大学院生物資源学研究科の論文