Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter
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概要
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Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.シイタケ・キチン合成酵素遺伝子プロモーターによってハイグロマイシン耐性遺伝子hphを発現させる発現ユニットを有する、シイタケ用の新規ベクター (pLCHS-hph と pChG-bar)を構築した。ランデムに選択した形質転換体におけるhphの発現をRT-PCR法によって確認した。2種類のベクターはシイタケの形質転換に有用であることが明らかとなった。
- Elsevierの論文
Elsevier | 論文
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