In vivo promoter analysis on refeeding response of hepatic sterol regulatory element-binding protein-1c expression
スポンサーリンク
概要
- 論文の詳細を見る
Sterol regulatory element-binding protein (SREBP)-1c is the master regulator of lipogenic gene expression in liver. The mRNA abundance of SREBP-1c is markedly induced when animals are refed after starvation, although the regulatory mechanism is so far unknown. To investigate the mechanism of refeeding response of SREBP-1c gene expression in vivo, we generated a transgenic mouse model that carries 2.2 kb promoter region fused to the luciferase reporter gene. These transgenic mice exhibited refeeding responses of the reporter in liver and adipose tissues with extents essentially identical to those of endogenous SREBP-1c mRNA. The same results were obtained from experiments using adenovirus-mediated SREBP-1c-promoter-luciferase fusion gene transduction to liver. These data demonstrate that the regulation of SREBP-1c gene expression is at the transcription level, and that the 2.2 kb 5′-flanking region is sufficient for this regulation. Moreover, when these transgenic or adenovirus-infected mice were placed on insulin-depleted state by streptozotocin treatment, the reporter expression was upregulated as strongly as in control mice, demonstrating that this regulation is not dominated by serum insulin level. These mice are the first models to provide the mechanistic insight into the transcriptional regulation of SREBP-1c gene in vivo.
- Elsevierの論文
著者
-
Shimano Hitoshi
Advanced Biomedical Applications Graduate School Of Comprehensive Human Sciences Univ. Of Tsukuba 1-
-
Kujiraoka T
Dep. Of Internal Medicine Graduate School Of Medicine Univ. Of Tokyo Tokyo 113-8655 Jpn
関連論文
- In vivo promoter analysis on refeeding response of hepatic sterol regulatory element-binding protein-1c expression
- Receptor-Type Protein Tyrosine Phosphatase ε (PTPεM) is a Negative Regulator of Insulin Signaling in Primary Hepatocytes and Liver
- A Kindred of Familial Acromegaly without Evidence for Linkage to MEN-1 Locus
- Molecular Mechanisms of Cholesteryl Ester Transfer Protein Deficiency in Japanese
- Serum Apolipoprotein J in Health, Coronary Heart Disease and Type 2 Diabetes Mellitus
- PE-436 Catalytically Inactive Lipoprotein Lipase Overexpression Increases Insulin Sensitivity in Mice(Diabetes/Obesity/Metabolic syndrome-4 (H) PE73,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Societ
- PE-044 Novel Regulation Pathway of the LDL Receptor Family for Migration of Vascular Smooth Muscle Cells(Atherosclerosis, basic-3 (H) PE8,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-198 LR11, an LDL Receptor Family Member, Inhibits Adipocyte Maturation through the Induced Stabilization of Beta-catenin(Diabetes/Obesity/Metabolic syndrome-2 (H) OJ33,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the J
- Resistin Causes Impaired Glucose Tolerance Accompanied with Decreased Insulin Sensitivities in Tissues of Mice Implanted with Resistin-overexpressing Cells (Diabetes/Obesity 6 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Enhanced Migration of Intimal Smooth Muscle Cells is Accompanied with Increased Expression of PDGF β-receptor in Explants from Rabbit Plaques (Atherosclerosis, Basic 7 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)