The Effect of Several Fixing Solutions on the Fixation Image and Staining of the Lilac Leaf （Syringa Vulgaris）
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概要
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The purpose of this study was to determine the effect of several killing agents on samples cut from the lilac leaves (Syringavzugaris). The leaves were cut in four different lengths to indicate the different treatments to be given to them. The killing solutions used were CRAF III，FAA II，Allen-Bouin II and a control with no kil1ingagent. A procedure for embedding in paraffin was used to provide one sample from each of the four treatments on each microscopic slide. The slides were stained with Safranine-O and Fast-Green; Safranine-O and Anilin Blue; Safranine-O and Bismarck Brown and Fast-Green; Safranine-O and Delafield's Hematoxylin; Conant's Triarch Quadruple and Heidenhain's Iron-Alum Hematoxyiin. The sections were examined tonote the effect of the four treatments on the fixation image and on the staining. From the standpoints of both the fixation image and staining，the findings indicate preference for treatment in the following order CRAF III，Allen-Bouin II，FAA II and no ki1ling agent.
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